BIOL 112 Lab 4

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Modified Objectives:

  • Alternation of Generations (haploid / diploid)
  • Evolutionary trend of gametophytes
  • Flower structure
  • 15 Microscope Slides
  • Pollination vs. Fertilization (double fertilization)
  • Visually distinguish among the following plant types:
    • Algae
    • Bryophyte (moss)
    • Ferns
    • Gymnosperms
    • Angiosperms

Supergroup Archaeplastida

Red algae, green algae, land plants

descended from an ancient protist that engulfed a photosynthetic cyanobacterium

Green Algae: relatives of land plants 2 Groups of green algae: chlorophyta and charophyta

Green algae show two adaptations for land environments:

  • Increased cellular organization and specialization
  • Shift from isogamous [vocab 1] to oogamous [vocab 2] sexual reproduction: egg is large and stationary, sperm are small and motile.

Group Chlorophyta

Asexual reproduction under favorable conditions, but sexually form a zygote to survive harsh conditions.

Individuals are haploid; zygote is diploid, but undergoes meiosis to form spores that germinate into algae

Representative individuals:

  Chlamydomonas Spirogyra Volvox Ulva
Organization Single-celled Filamentous (attached end-to-end) Colonial (primary and daughter) Multicellular "blades" w. specialized structures
Asexual Reproduction mitosis mitosis and fragmentation [vocab 3] mitosis — forms inner daughter colony
Gametes (Sexual Reproduction) Isogamous Isogamous (zygospores formed internally prior to conjugation) Oogamous (zygospores formed inside colony and released when colony disintegrates) Isogametes; isogamous (true alternation of generations w. distinct haploid/gametophyte and diploid/sporophyte stages)
Flagella Two Flagellated gametes Many, synchronized; flagellated sperm Flagellated isogametes
Dominant Stage Haploid (reduced diploid stage) Haploid (reduced diploid stage) Haploid (reduced diploid stage) Split betw. haploid and diploid thallus


Group Charophyta

stoneworts

closest-living algae with algal and plant-like characteristics


Alternation of Generations

  1. haploid gametophyte produces haploid gametes by mitosis
  2. gametes merge to form diploid zygote
  3. zygote undergoes mitosis and grows into a diploid sporophyte
  4. sporophyte produces haploid spores by meiosis
  5. spores undergo mitosis to form a gametophyte (cycle starts over)


Group Bryophyta

Mosses, liverworts, and hornworts

homospory
male and female spores look identical
heterospory
male gametophytes come from microspores
female gametophytes come from macrospores
  • Lack vascular tissues (no true roots, stems, or leaves)
  • Flagellated sperm require moist environment
  • Dominant haploid gametophyte thallus and reduced diploid sporophyte

Dioecious plants (separate "male" and "female" plants):

  • Females have archegonia
  • Males have antheridia


Group Pterophyta

Ferns; seedless vascular plants

Development of vascular tissues (carry water and nutrients throughout plants)

  • Stem is called stolon
  • Roots are called Rhizomes

Still depend on water to transport flagellated sperm to egg

Dominant form is the diploid sporophyte; diminished haploid gametophyte prothallus that can be monoecious (male and female parts—antheridia and archegonia—on same structure).

Zygote develops attached to gametophyte


Vascular Plants

gymnosperms
"naked" (gymno-) "seed" (-sperm)
cone-bearing
angiosperms
seed enclosed in fruit
flowering plants

Seeds allow for following adaptations:

  • survive harsher conditions (cold, drought)
  • small multicellular sporophyte can remain dormant
  • embryo protected by seed coat and surrounded by food supply
  • facilitated dispersal
  • female gametophyte protected inside diploid sporangium


Gymnosperms

Cycads (dioecious)

Heterosporous:

  • microspores made on microstrobilus (cone)
  • macrospores made on macrostrobilus

Ginkgophyta (also dioecious) do not have macrostrobiili, but two bare ovules

Coniferophyta (monoecious): Female macrostrobli grow at top of tree, while male microstrobili grow on bottom (prevents self-pollination)

Pollination v. Fertilization

Pollination
when pollen lands on a female gametophyte
Fertilization
when sperm contained within pollen reaches egg and fuses


Angiosperms

Ovary located deep inside flower

Vocabulary

  1. isogamous: male and female gametes appear identical
  2. oogamous: male and female gametes appear different
  3. fragmentation: a broken part of an organism can regenerate the rest of the lost parts to form a new organism