BIOL 111 Lab Exercise 6

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End Lab Practical 1 Content

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Glycolysis

  • Occurs in Cytosol
  • Anaerobic

Krebs Cycle

  • Occurs in Mitochondria
  • Aerobic (requires O2)

Oxidative Phosphorylation

a.k.a. Electron Transport Chain LOTS of ATP.

  • DPIP, normally a blue color, was used as electron acceptor in experiment.
  • Reduced to DPIPH2, which is clear. (Absorbance decreases)

TTC tests for working respiration

Inhibitors

Competetive
binds to active site of enzyme
Malonate was inhibitor in experiment
Noncompetetive
binds to other place on enzyme to change shape of active site

OIL RIG: Oxidation Is Loss, Reduction Is Gain